ImmunoGlo™ MLC

Bioluminescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay


Buy ImmunoGlo™ MLC Assay Kits

Bioluminescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay Kit 


  • One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Cellular immune response.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.
  • Non-radioactive assay incorporating proven ATP bioluminescence technology, to replace all radioactive MLC/MLR assays.
  • Instrument-based, quantitative and fully standardized readout procedure.
  • 10 - 100 times more sensitive than WST-1, CSFE and other absorbance or fluorescence assays.
  • Complete flexibility to use virtually any MLC/MLR protocol.
  • Reliably compare results over time.
  • Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.

Principle of ATP Bioluminescence Cell Proliferation Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
  • Applicable to multiple species.
  • Peripheral blood mononuclear cells (PBMC)
  • Lymphocytes and lymphocyte sub-populations from a variety of sources
  • Lymphocyte cell lines
  • Other potential immunoreactive cell types

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ImmunoGlo™ MLC Assay Kits

Assay Type Catalog Number Number of Plates
ImmunoGlo™ MLC KM1-MLC-1 1

For Research Use Only. Not for clinical diagnostic use.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • Mitomycin-C for one-way MLC/MLR    
  • ATP standard    
  • ATP high and low controls    
  • ATP Enumeration Reagent    
  • Sterile, 96-well culture plate(s)    
  • Non-sterile, 96-well plate(s)    
  • Sterile adhesive plate cover foils to maintain sterility of unused wells    

ImmunoGlo™ MLC Assay Manual

Bioluminescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay